If you mean just the cylinder head, they typically don't have an issue coming loose. If you have removed the four nuts and washers that hold it to the cylinder, it should come off relatively easily. It's possible if you didn't get the washers off, they could have been mashed into the threads of the studs a bit, creating a bit of a lock. You can try a screwdriver or other pry bar between the bottom head fin and the top of the cylinder, but be very careful that you get it deep in between and pry as little as possible against the outer edges of the fins or you may crack them. Otherwise, I'd screw the nuts back on the studs a few threads and hold the engine up by the head, and use them to tap against to drive the cylinder away from the head. It would probably take two people.
Another possibility is that if someone used a strong sealer instead of a head gasket, maybe it is in effect "glued" on. In that case heating to 450-500° F will reduce the grip and not hurt anything else.